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Equipment

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The accredited Laboratory of the Institute for Oliveculture at ZRS Koper is equipped with state-of-the-art analytical equipment that ensures high quality, reliability, and traceability of measurements at the national and international levels.

The equipment is used for chemical and sensory analyses of olive oil and other plant-based products, as well as for the development and validation of new analytical methods. Modern systems enable the precise quantification of bioactive compounds, the detection of contaminants such as MOSH and MOAH, and the verification of the quality and authenticity of olive oil and other foods.

High Performance Liquid Chromatograph (UHPLC)

  1. Mobile-phase degasser
  2. Gradient pump with a flow rate from 0.05 mL/min to 5 mL/min at pressures up to 600 bar
  3. Automatic sampler and injector with a volume range from 0.1 µL to 100 µL
  4. Column thermostat with temperature control from 10 °C below ambient temperature to 65 °C
  5. UV detector with a 1024-diode array (DAD) covering the range from 190 nm to 640 nm

Tandem mass spectrometer in a quadrupole–hexapole–quadrupole (QQQ) configuration

  1. Mass range from 10 amu to 2,200 amu
  2. At least 500 MRM transitions per method segment
  3. Polarity switching time of less than 30 ms
  4. Mass accuracy better than 0.01% in the range from 1,000 amu to 2,000 amu
  5. Linear dynamic range ≥ 6 decades
  6. Ion source: atmospheric-pressure electrospray ionization (AP-ESI)
  7. Integrated system for automatic ion-optics optimization and mass-axis calibration with automatic infusion of a calibration standard (AP-ESI ion source)
  8. The QQQ mass spectrometer supports the following operating modes:
    1. MS scanning and MS/MS product-ion scanning
    2. MRM measurement of multiple-ion MS/MS transitions
    3. SIM measurement
    4. MS/MS scanning of neutral losses/gains
    5. precursor-ion scanning.
  9. Nitrogen generator for supplying the ESI mass spectrometer (output flow 30 L/min at 6.5 bar)

All equipment includes installation qualification (IQ) for the software, HPLC, and QQQ systems in accordance with GLP guidelines, as well as operational/performance qualification (OQ/PV) of the software, HPLC, and QQQ systems in accordance with GLP guidelines.

Date received: December 3, 2012

Date installed: December 10, 2012

Date of commissioning: December 17, 2012

QQQ

QQQ (Photo: LAB IZO Archive)

UHPLC naprava

UHPLC (Photo: LAB IZO Archive)

UHPLC + QQQ

UHPLC + QQQ (Photo: LAB IZO Archive)

Source of funding: Utilization of Cohesion Policy funds at the Metrology Institute of the Republic of Slovenia (MIRS)
Owner: Republic of Slovenia, Ministry of Economic Development and Technology, Metrology Institute of the Republic of Slovenia, free lease to the designated national standard holder under contract C2132-13-000017 dated February 15, 2013

Agilent 1260 Infinity High Performance Liquid Chromatograph with 6420 Triple Quad LC/MS Tandem Mass Detector

Manufacturer: Agilent Technologies

1290 Thermostat: Model G1330B, Serial no.: DEBAK11089, MIRS designation: 2132020036

Modul: 1260 ASL: Model G1329B, Serial no.: DEAAC15052, MIRS designation: 2132020035

Modul: 1260 TCC: Model G1316A, Serial no.: DEACN16395, MIRS designation: 2132020037

Modul: 1260 DAD: Model G4212B, Serial no.: DEAA304076, MIRS designation: 2132020038

Modul: 1260 Bin Pump: Model G1312B , Serial no.: DACB04528, MIRS designation: 2132020034

Modul: 1260 HiP Degasser: Model: G4225A, Serial no.: JPAAA01558, MIRS designation: 2132020033

Detektor 6420 Triple Quad LC/MS + API ESI: Model: G6420A, Serial no.: SG12377007, Model: G1948B, Serial no.: SG12389030, MIRS designation: 2132020030

Pump P/N G 1960-80040: Model: P499241M002, Serial no.: IT1237D024 (part of the 6420 detector)

Nitrogen generator NM32LA (Manufacturer: Peak Scientific), Serial no.: A12-11-262, MIRS designation: 2132020039

  • Electronic Pneumatic Control (EPC) with ALS and LAN interface, up to 450 °C, heating rate up to 120 °C/min
  • Flame Ionization Detector (FID) for capillary columns
  • Agilent 7693A Automatic Liquid Sampler (ALS), 16-position turret, syringe support for 1–100 µL syringes
  • Agilent 7693A Automatic Sample Tray with heater, agitator, and barcode reader for individual vials, 150-vial capacity, 3 removable trays

Date received: September 18, 2018

Date installed: September 20, 2018

Date of commissioning: October 1, 2018

Olive oil analysis instrument

Source of funding: Utilization of Cohesion Policy funds at the Metrology Institute of the Republic of Slovenia (MIRS). State aid and insurance reimbursement.
Replacement equipment for equipment destroyed in a fire, acquired as part of the Metrology System Infrastructure project – Quantity of Substances /Plant-Based Food/Olive Oil in 2012.
Owner: Republic of Slovenia, Ministry of Economic Development and Technology, Metrology Institute of the Republic of Slovenia, free lease to the designated national standard holder under contract C2132-13-000017 dated February 15, 2013.

  • Electronic Pneumatic Control (EPC) with ALS and LAN interface, up to 450 °C, heating rate up to 120 °C/min
  • Flame Ionization Detector (FID) for capillary columns
  • GERSTEL MultiPurpose Sampler (MPS) Robotic XL for liquid injection and headspace sampling
  • MPS Robotic SPME Fiber Conditioning Station

Date received: August 2, 2018

Date of installation: August 31, 2018

Date of commissioning:

Funding: ARRS Package 17

Agilent 7890B Gas Chromatograph (Agilent Technologies) with GERSTEL Robotic Sampler

Source of funding: State aid and reimbursement from the insurance company for replacement equipment following the destruction of ZRS Koper’s equipment in a fire. Funding: ARRS Package 17

BÜCHI Rotavapor R-300 (BÜCHI Labortechnik AG)

Specifications:

  • Rotation speed: 10–280 rpm
  • Maximum evaporation flask capacity: 3 kg
  • Heating bath: 5 L; temperature range 20–220 °C
  • Manual lift mechanism
  • Integrated display showing set and actual temperature, rotation speed, and lift position

BÜCHI Rotavapor R-300 Rotary Evaporator with Cooling System (BÜCHI Labortechnik AG)
The BÜCHI Rotavapor R-300 is connected to a JULABO F250 recirculating cooling system.

JULABO F250 Cooling System – Technical Specifications:

  • Operating temperature range: −10 to +40 °C
  • Temperature stability: 0.5 °C
  • Cooling capacity (at 20 °C): 0.25 kW
  • Pump flow rate: 15 L/min
  • Pump pressure: 0.35 bar
BÜCHI Rotavapor R-300 Rotary Evaporator with Cooling System

BÜCHI Rotavapor R-300 Rotary Evaporator with Cooling System,

Date received: July 18, 2018

Date of installation: July 18, 2018

Date of commissioning: August 1, 2018

Funding: ARRS Package 17

FT-NIR Spectrometer MPA II, Bruker (manufacturer: Bruker Optics)

The MPA II is a powerful tool for developing sophisticated calibration methods for laboratory or process needs, as well as an easy-to-use instrument for routine QA/QC work. Its modular technology allows individual configuration for each specific analytical task.

Features:

  • Enables transmission measurements for liquid and solid samples, measurements using the integrating sphere, and measurements of solid and liquid substances via a fiber-optic probe.
  • A selection of software-guided tools and PerformanceGuard™ technology for monitoring spectral quality.
  • Long-life light source with enhanced robustness and low maintenance costs.
  • Durable solid-state laser with the highest wavenumber accuracy and long service life.
  • Direct calibration transfer (to and from all existing Bruker NIR spectrometers).
  • A wide range of pre-configured calibration packages suitable for numerous industries.
  • RockSolid™ interferometer with permanently aligned optics, non-sensitive to vibration.
  • Fully automated routine tests for validation in accordance with cGMP (OQ, PQ).
  • Compliant with 21 CFR Part 11 and validation according to EU, JP and US Pharmacopoeia.

Specifications:

SPECTROMETER

  • Spectral range: sample compartment: 11,500 – 4,000 cm⁻¹; fiber optic module: 11,500 – 4,000 cm⁻¹; integrating sphere; transmission unit: 11,500 – 5,800 cm⁻¹.
  • Spectral resolution: max. 2 cm⁻¹.
  • Wave number accuracy: 0.1 cm⁻¹.
  • Wave number repeatability: 0.006 cm⁻¹.
  • Photometric accuracy: 0.1 % T.
  • Photometric linearity: 1.00 ± 0.05 (slope); 0.00 ± 0.05 (offset).
  • Measurement speed: up to 5 scans/second at 8 cm⁻¹ resolution.

OPTICAL COMPONENTS OF THE SPECTROMETER

  • Source: tungsten, air-cooled (20 W).
  • Beamsplitter: quartz with proprietary coating.
  • Interferometer: RockSolid™ interferometer with gold-coated mirrors, permanently aligned, high stability.
  • Scanner: frictionless bearings, choice of 4 different speeds — 3.75 kHz, 7 kHz, 15 kHz, 30 kHz.
  • Detector:

Sample compartment, fiber optic module, integrating sphere: high-sensitivity, thermoelectrically cooled InGaAs detector.

Transmission unit: high-sensitivity InGaAs detector, operating at room temperature.

Spectrometer

Dri-Block Heater DB200/3, manufacturer: Techne

Specifications:

  • Working range: 5 to 200 °C
  • Set-temperature resolution: 0.1 °C
  • Accuracy at 37 °C: ≤ 1 °C
  • Accuracy at 100 °C: ≤ 1 °C
  • Temperature stability at 37 °C: ≤ 0.1 °C
  • Temperature stability at 100 °C: ≤ 0.15 °C
  • Maximum temperature difference within the same block: < 0.2 °C at 37 °C and < 0.2 °C at 100 °C
  • Maximum temperature difference between blocks: < 0.5 °C at 37 °C and < 1.5 °C at 100 °C
  • Heating time from 25 to 100 °C: 7 min
  • Heating time from 25 to 200 °C: 19 min
Dri-Block Heater DB200/3